Monday, June 21, 2010

Glowing mushrooms in Brazil

http://blog.makezine.com/archive/2006/11/new_glowing_mushrooms_fou.html

Tuesday, March 2, 2010

Dinoflagellate

These are the guys that photoemit when stirred up:

http://en.wikipedia.org/wiki/Dinoflagellate

Optimal conditions for photoemission

Continuous cultivation of the bioluminescent bacterium, Vibrio fischeri NRRL-B-11177, in a fermenter provided a constant supply of bacteria in exponential growth phase. These bacteria may be used as stable bioindicators to measure perturbation of the metabolic state by physical and chemical agents. However, optimization of light emission necessitates careful choice of growth medium and culture operating conditions. Optimized conditions that supported long-term cultivation of V. fischeri NRRL-B-11177 with stable intense bioluminescence were: a dilution rate of 0.08–0.09 h− 1, air supply of 600 ml min− 1, stirring of 300–350 rpm at a constant incubation temperature within the range of 20 to 26 °C and pH 7.8. These conditions were as successful in a 500-ml as in a 10-ml fermenter. This system provided a reliable long-term (more than 1 month) continuous culture facility for the reproducible measurement of perturbation of V. fischeri by monitoring changes in luminescence.

Full article.


Saturday, February 27, 2010

books

Bioluminescence: Camouflage, Biophoton, Foxfire (bioluminescence), Bioluminescence imaging, List of light sources, Milky seas effect, Light, Organism, Hybrid word, Greek language

http://www.amazon.com/Bioluminescence-Camouflage-Biophoton-bioluminescence-Organism/dp/6130219199/ref=sr_1_6?ie=UTF8&s=books&qid=1267307263&sr=1-6


Aglow in the Dark: The Revolutionary Science of Biofluorescence

http://www.amazon.com/Aglow-Dark-Revolutionary-Science-Biofluorescence/dp/0674024133/ref=sr_1_5?ie=UTF8&s=books&qid=1267307504&sr=1-5


Glowing Genes: A Revolution In Biotechnology

http://www.amazon.com/Glowing-Genes-Biotechnology-Marc-Zimmer/dp/1591022533/ref=sr_1_8?ie=UTF8&s=books&qid=1267307527&sr=1-8


Bioluminescence: Methods and Protocols (Methods in Molecular Biology)

http://www.amazon.com/Bioluminescence-Methods-Protocols-Molecular-Biology/dp/1603273204/ref=sr_1_5?ie=UTF8&s=books&qid=1267307553&sr=1-5

Bioluminesence Patterns

Text from the National Science Foundation website[1]:

"This arch was composed with petri dishes "painted" with bioluminescent bacteria. The piece--approximately 9 feet high by 5 feet wide--was installed in December 2002 at the O'Malley Library, Manhattan College, Riverdale, NY. The painting was created as part of the Bioglyphs project, composed of participants from Montana State University-Bozeman's Center for Biofilm Engineering (CBE) and School of Art, in collaboration with environmental engineering students of Dr. Robert Sharp at Manhattan College. For more information about the project, visit the Bioglyphs website at http://www.erc.montana.edu/Bioglyphs/. (Note: The CBE was established in 1990 as an NSF Engineering Research Center at Montana State University--Bozeman, to foster a new approach to university engineering and science education.) [See related images: Bioluminescent Bacteria Glow Brightly and Bioglyphs Secrets Unveiled.]

More about this Image: Bioglyphs is an art and science collaboration initiated in 2002 by members of the Center for Biofilm Engineering (CBE) and the Montana State University School of Art. Two Bioglyphs exhibitions of living bioluminescent paintings were created by teams of student and staff artists, scientists, and engineers in 2002.

Microorganisms live all around us but we are rarely aware of their presence. The Bioglyphs exhibition allowed viewers to have direct sensory contact with a microscopic organism. Scientists are unsure of the exact identity of the bioluminescent organisms that were used in the exhibit but they are believed to be single-celled, marine-environment bacterial isolate, probably of the Vibrio species. These bacteria only grow on a high-salt medium at relatively low temperatures--considerably lower than the internal temperature of the human body. Like many marine organisms, they produce blue light through a chemical reaction. Other Vibrio species such as Vibrio fischeri, will produce light after a certain number of organisms have accumulated. Why the light is produced by communities rather than by a single organism in these species is unknown, but the phenomenon raises questions about the nature of communal response and interaction.

In order to obtain the bacteria used in the exhibit, scientists from the CBE prepared plates with a nutrient medium that would sustain the bacteria for a limited period of time. Successful growth however, will depend on numerous factors, not all of which can be controlled. How the bacteria will respond to an environment created for them is inherently unpredictable.

When the bacteria are transferred to petri dishes, they are invisible but within 24 hours, they rapidly multiply and begin to emit a blue light. Over the course of several days, light production peaks and then begins to decline, as available nutrient is used up. This life cycle heightens our awareness of resource limitations as well as species-interdependency.


Friday, February 26, 2010

Exoloratorium project

http://press.exploratorium.edu/inspired-by-life-march-2010/

Bioluminescent Algae in Australia

http://www.youtube.com/watch?v=EN1Yxq8KMsw

Bioluminescent Bacterium Kit

http://www.carolina.com/product/living+organisms/prokaryotes/bacteria+kits/bioluminescent+bacterium+kit.do?sortby=ourPicks

Instructables project for making biolumiescent algae

http://www.instructables.com/id/Grow-Your-Own-Bioluminescent-Algae/

MATERIALS:

+ Growing Container
Clear and shallow with lots of surface area
?

+ Sea Salt
http://www.petsmart.com/product/index.jsp?productId=2754897
$4.99

+ Grow light and timer
Available from Walmart
Flourescent light and rack and timer
About $10

+ Algae
http://empco.org/edu/index.php/live-algae/bioluminescent-algae.html?SID=d1766a4abbf23a488bd6f237199ee71f
$16.25

+ Starter culture solution
http://empco.org/edu/index.php/growth-media/marine-media/l1-marine-growth-medium.html
$5.49 each (avoid need for salt?)

+ Micro Algae Grow
https://3kserver7.com/~frank/secure/agora.cgi?cart_id=7661857.11433*N35tF1&product=MICRO_MACRO_NUTRIENTS
$5-$27

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